FD Rapid GolgiStain Kit

Introduction

Golgi-Cox impregnation1, 2 has been one of the most effective techniques for study the normal and abnormal morphology of neurons and glia. Using the Golgi technique, subtle morphological alterations in neuronal and dendritic dendrites. Spines have been discovered in the brains of drug-treated animals, as well as in the post mortem brains of patients with neurological diseases3, 4. However, the reliability and the slow process of Golgi staining have been major obstacles to the generalization application of this technique.

The FD Rapid GolgiStain ™ Kit is designed according to the principle of methods described by Ramón-Moliner2 and Glaser and Van der Loos5. This kit not only has drastically improved and simplified the Golgi-Cox technique but has also been shown to be extremely reliable and sensitive for demonstrating morphological details of neurons and glia, especially dendritic spines. The FD Rapid GolgiStain ™ Kit has been tested extensively in the brains of various species of animals, as well as in specimens of Post-mortem human brains (for photo samples and references with this kit, visit our website at www.fdneurotech.com).

Materials required but not included

  1. Bidistilled or deionized water
  2. Plastic/glass tubes or vials
  3. Histological material and equipment:
  • Gelatin-coated microscope slides
  • Coverslips
  • Staining jars
  • Ethanol
  • Xylene or xylene substitutes
  • Resinous mounting medium (eg Permount®)
  • An optical microscope.

Safety and handling precautions

  • The FD Rapid GolgiStain Kit is intended for in vitro research use only and not for drugs, diagnostics, or other uses.
  • The kit contains reagents that are toxic and harmful in contact with the skin or by inhalation and can be fatal if swallowed. Do not pipet by mouth. Avoid inhalation and skin and eye contact. In case of contact, wash immediately with generous amounts of water and seek medical attention. In case of ingestion, wash your mouth with water and call a doctor immediately.
  • Perform the experiment under a chemical hood. Wear suitable protective clothing, gloves, and eye/face protection while handling kit reagents. Handwashing thoroughly after conducting the experiment.

Tissue preparation

  • All containers (preferably plastic) to be used should be cleaned and rinsed with distilled water.
  • Do not use metal implements when Solutions A and B are present.
  • Keep containers tightly closed at all times.
  • All tissues treated with Solutions A and B, including tissue sections, must be protected from light.
  • The following procedure should be performed at room temperature unless specifically indicated.

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