Pure Galanthus nivalis lectin, 5mg

Properties

Quality level: 200

Form: saline suspension

Labeling extension: 2-4 mg of protein per mL

Matrix: 4% cross-linked agarose beads

Ability

5-10 mg / ml binding capacity (yeast mannan) (pH 7.2 at 25 ° C)

Conjugate: agarose conjugate

Storage temperature: 2-8 ° C

Description

Pure GNL / GNA lectin is isolated from bluebell bulbs, Galanthus nivalis and purified by affinity chromatography. GNL consists of four identical subunits with a molecular weight of 50,000 and isoelectric points between pH 3.5 and 4. This lectin is known to agglutinate rabbit erythrocytes but not human erythrocytes. LNG contains little or no carbohydrates and specifically binds to murine IgM immunoglobulin and human α 2-macroglobulin, but not to alpha-bound glucose (unlike most mannose-binding lectins).

Structures containing mannose residues (α-1,3) are preferred for binding and elute a 0.2 M concentration of Meα-mannose. GNL is useful for HIV research, applicable for blood cell agglutination studies, and has established a model system to help understand the molecular basis of how proteins recognize carbohydrates. This product comes in the form of a lyophilized powder and is stable for more than five years from the date of production when stored below -20 C. EEL does not require metal ions to bind.

Packaging

2 mL in a glass bottle

Biochemical / Physiological Actions

GNL agglutinates rabbit erythrocytes; human erythrocytes do not agglutinate. The specificity of LNG sugar is directed at the non-reducing end of the terminal α-D-mannosyl residue of glycoconjugates.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide.

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