The purpose of the current work is to develop a laboratory-scaled methodology for an on-demand provide of semen from the ocean urchin Paracentrotus lividus. Firstly, sea urchin specimens had been acclimatized to the long-term rearing in a recirculating aquaculture system and gonad maturation was obtained below managed circumstances. Semen samples had been then collected from mature sea urchins and cryopreserved. Lastly, post-thawing motility was evaluated, to confirm whether or not the cryopreserved semen had maintained sufficient viability for use in laboratory actions.
The post-thawing motility parameters remained fairly unchanged for as much as 60 min after activation; furthermore, the semen even retained the power of motility activation for 60 min after thawing. This motility sample makes using cryopreserved semen a possible possibility in spermiotoxicity bioassays. The preliminary ecotoxicity take a look at, carried out utilizing motility parameters as endpoints, confirmed sensitivity ranges to cadmium falling in the identical order of magnitude as these recorded for recent sea urchin semen and for cryopreserved sea bream semen. . Subsequently, semen samples produced and saved in response to the developed methodology described on this paper, could be thought of a promising and sustainable different to these collected from mature sea urchins harvested within the discipline.
Economical bluetooth low energy-based telemetry system with mixed information processing methodology for long-term laboratory animal monitoring for organic rhythm analysis
A telemetry system primarily based on Bluetooth Low Power (BLE) was constructed to concurrently gather locomotor exercise and physiological alerts of small animal cohorts for circadian rhythm experiments; it consists of miniature transmitters and cell phone with custom-made App. The continual sampling alerts obtained from the 3-axis acceleration and temperature sensors within the transmitters are despatched to the cell phone in real-time by Web of Issues (IoT) for momentary storage after which imported into the pc for abstract and rhythm evaluation by the overall open-source mathematical software program.
Not like costly and sophisticated industrial telemetry techniques with industrial wi-fi requirements, no particular information receivers and software program are wanted. In our validation experiment, six rats had been divided into two teams below pure darkish and light-dark cycles. For 2 consecutive weeks, the transmitter mounted on the top of the rat-recorded locomotor exercise, pores and skin temperature, and ambient temperature of every rat at a frequency of 6 Hz.
After processing with Native Weighted Regression Scatter Smoothing (LOWESS) and Quick Fourier Rework (FFT) filtering, single cosinor and multi-components cosinor had been then used to evaluate and characterize the circadian rhythm. The outcomes confirmed that the rhythm values of the 2 teams of rats coincided with the corresponding light-dark cycle, and that the system was sturdy to information loss and error from BLE communication failures. Subsequently, the proposed system gives a lightweight framework for long-term circadian rhythm monitoring in free-moving rodents to additional simplify and promote experimental chronobiology animal research.
Scholar-Scientist Curriculum: Integrating Inquiry-Primarily based Analysis Experiences and Skilled Improvement Actions into an Introductory Biology Laboratory Course
We designed a 16-week scaffolded student-scientist curriculum utilizing inquiry-based analysis experiences built-in with skilled improvement actions. This curriculum was carried out to show undergraduate college students enrolled in an introduction to biology course about enzyme exercise, biochemical reactions, and alcohol fermentation. Whereas working by the curriculum, college students accomplished your entire scientific course of by planning experiments, sustaining laboratory journals, analyzing and deciphering information, peer-reviewing analysis proposals, and producing and presenting a poster.
The general consequence was for college kids to finish a multiweek, collaborative, student-scientist venture utilizing Saccharomyces cerevisiae because the mannequin organism. Scholar studying outcomes had been evaluated utilizing formative assessments (post-Analysis on the Built-in Science Curriculum survey and peer- and self-reflection worksheets) and summative assessments (pre/publish assessments and project grades).
Outcomes indicated that greater than 50% of the scholars scored 70% or larger on the collaborative student-scientist venture, demonstrated a number of self-reported studying features in scientific ideas and expertise, and reported they’d advocate this laboratory course to their friends. By offering the chance for college kids to hold out your entire scientific course of, this curriculum enhanced their technical, analytical, and communication expertise.
Analysis Ethics Committees in Laboratory Medication
Biomedical analysis that includes human topics requires compliance with moral rules and pointers. The moral and scientific requirements of analysis have been completely mentioned by worldwide moral pointers and declarations. Compliance with these ensures the autonomy, dignity and well-being of analysis topics; in addition to the integrity and credibility of analysis outcomes. Analysis ethics committees (RECs) are mandated to make sure that analysis proposals are scientifically sound and moral.
On this evaluate, we outline RECs in laboratory drugs and describe their function primarily based on the examination of the necessities of moral analysis; focus on specific moral points that come up in laboratory drugs analysis utilizing organic samples, what challenges they face and the way they’ll guarantee the standard of their evaluate. RECs must be put right into a broader framework that ensures institutional governance with steady analysis and auditing that guarantee the standard of moral evaluate.
A Survey of Laboratory Animal Veterinarians Relating to Mouse Welfare in Biomedical Analysis
The standard of analysis animal welfare is undeniably linked to the standard of scientific outcomes generated from the animals.Though mice are essentially the most generally used mammalian species in biomedical analysis, little data is out there aboutwhat components ought to be thought of to advertise future progress. To deal with this subject, the Animal Welfare Committee of theAmerican Society of Laboratory Animal Practitioners (ASLAP) surveyed laboratory animal veterinarians to acquire theiropinions concerning the welfare of mice and to contemplate the roles of 5 components that considerably have an effect on animal welfare in biomedical analysis: husbandry, medical care, experimental use, regulatory oversight, and coaching.
 Anti-RPSA Alexa Fluor® 488 |
|
A4-829-C100 |
ExBio |
0.1 mg |
EUR 310 |
) Anti-CD40 antibody (Alexa-fluor 488) |
|
STJ170000 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin |
) Anti-LAMP3 antibody (Alexa-fluor 488) |
|
STJ170004 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-LAMP3 antibody (Alexa-fluor 546) |
|
STJ170005 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-LAMP3 antibody (Alexa-fluor 647) |
|
STJ170006 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-IL3RA antibody (Alexa-fluor 488) |
|
STJ170009 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-IL3RA antibody (Alexa-fluor 546) |
|
STJ170010 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-IL3RA antibody (Alexa-fluor 647) |
|
STJ170011 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-CD207 antibody (Alexa-fluor 488) |
|
STJ170014 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-CD207 antibody (Alexa-fluor 546) |
|
STJ170015 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-CD207 antibody (Alexa-fluor 647) |
|
STJ170016 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-IL7R antibody (Alexa-fluor 488) |
|
STJ170020 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
) Anti-IL7R antibody (Alexa-fluor 546) |
|
STJ170021 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
) Anti-IL7R antibody (Alexa-fluor 647) |
|
STJ170022 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
) Goat anti Mouse IgG1 (Alexa Fluor 488) |
|
43R-1649 |
Fitzgerald |
500 ug |
EUR 570 |
|
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
 Anti-Hu CD16 Alexa Fluor® 488 |
|
A4-646-T100 |
ExBio |
100 tests |
EUR 269 |
 Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
|
1795 |
AAT Bioquest |
10 mg |
EUR 393 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
) Mouse IgG1-Alexa 555 conjugate (isotype control) |
|
20102-101-A555 |
Alpha Diagnostics |
50 Tests |
EUR 263 |
 AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
|
23150 |
AAT Bioquest |
300 Tests |
EUR 306 |
- R-phrase: R23, R24, R25
- H-Phrase: H301, H311, H331
- Symbol for dangerous compounds: T
- UNSPEC Code: 12352200
|
 AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
|
23153 |
AAT Bioquest |
300 Tests |
EUR 306 |
- R-phrase: R23, R24, R25
- H-Phrase: H301, H311, H331
- Symbol for dangerous compounds: T
- UNSPEC Code: 12352200
|
 AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
|
23158 |
AAT Bioquest |
300 Tests |
EUR 306 |
- R-phrase: R23, R24, R25
- H-Phrase: H301, H311, H331
- Symbol for dangerous compounds: T
- UNSPEC Code: 12352200
|
 AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
|
16890 |
AAT Bioquest |
1 mg |
EUR 176 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
|
16891 |
AAT Bioquest |
1 mg |
EUR 176 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
|
16892 |
AAT Bioquest |
1 mg |
EUR 176 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID005AF |
Fitzgerald |
500 ug |
EUR 338 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 (Alexa Fluor 594)) Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 364 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
|
43R-ID028AF |
Fitzgerald |
500 ug |
EUR 430 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 462 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 (Alexa Fluor 594)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-ID056AF |
Fitzgerald |
500 ug |
EUR 343 |
|
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
 (Alexa Fluor 647)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
|
43R-ID060AF |
Fitzgerald |
300 ug |
EUR 425 |
|
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
 (Alexa Fluor 594)) Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-IR016AF |
Fitzgerald |
1 mg |
EUR 281 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
 Anti-Hu CD72 Alexa Fluor® 488 |
|
A4-310-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Bov CD9 Alexa Fluor® 488 |
|
A4-354-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Anti-Hu CD30 Alexa Fluor® 700 |
|
A7-455-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD94 Alexa Fluor® 700 |
|
A7-727-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD56 Alexa Fluor® 700 |
|
A7-789-T100 |
ExBio |
100 tests |
EUR 269 |
 Alexa Fluor 594–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
|
S0005 |
Affbiotech |
200ul |
EUR 376 |
 Alexa Fluor 594–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
|
S0006 |
Affbiotech |
200ul |
EUR 376 |
 Alexa Fluor 647–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|
S0013 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 647–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|
S0014 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|
S0017 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
|
S0018 |
Affbiotech |
200ul |
EUR 304 |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|
43R-ID012AF |
Fitzgerald |
300 ug |
EUR 410 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
, Alexa Fluor® 594 Conjugated) Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
|
Ab8011-001 |
GenDepot |
1mg |
EUR 334 |
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|
Ab8032-001 |
GenDepot |
0.5mg |
EUR 435 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
|
A4-686-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
|
A4-712-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
|
A4-730-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
|
A4-737-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
|
A6-686-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
|
A6-712-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
|
A6-730-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
|
A6-737-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Monoclonal Antibody (104G4) (Alexa Fluor<sup>®</sup> 488)) Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
|
M09406 |
BosterBio |
100ug |
EUR 565 |
|
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
 Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor<sup>®</sup> 488)) Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
|
M02316 |
BosterBio |
100ug |
EUR 580 |
|
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|
50336 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
-Alexa 594 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
|
50337 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
 AG 555 |
|
B6377-10 |
ApexBio |
10 mg |
EUR 144 |
|
Description: AG 555 is a potent and selective inhibitor of EGFR with IC50 value of 0.7 ?M. The epidermal growth factor receptor (EGFR) is the cell-surface receptor for epidermal growth factor and plays an important role in tumor invasion and cancer cell proliferation. |
AG 555 |
|
B6377-25 |
ApexBio |
25 mg |
EUR 276 |
|
Description: AG 555 is a potent and selective inhibitor of EGFR with IC50 value of 0.7 ?M. The epidermal growth factor receptor (EGFR) is the cell-surface receptor for epidermal growth factor and plays an important role in tumor invasion and cancer cell proliferation. |
AG 555 |
|
B6377-50 |
ApexBio |
50 mg |
EUR 447 |
|
Description: AG 555 is a potent and selective inhibitor of EGFR with IC50 value of 0.7 ?M. The epidermal growth factor receptor (EGFR) is the cell-surface receptor for epidermal growth factor and plays an important role in tumor invasion and cancer cell proliferation. |
 MCC-555 |
|
C4892-1 |
ApexBio |
1 mg |
EUR 158 |
|
Description: MCC-555, also known as RWJ-241947, is a novel peroxisome proliferator?activated receptor ? ligand [1]. The PPAR? receptors mainly express in adipose tissue, colon and macrophages involved in regulating fatty acid storage and glucose metabolism. |
MCC-555 |
|
C4892-10 |
ApexBio |
10 mg |
EUR 918 |
|
Description: MCC-555, also known as RWJ-241947, is a novel peroxisome proliferator?activated receptor ? ligand [1]. The PPAR? receptors mainly express in adipose tissue, colon and macrophages involved in regulating fatty acid storage and glucose metabolism. |
MCC-555 |
|
C4892-5 |
ApexBio |
5 mg |
EUR 538 |
|
Description: MCC-555, also known as RWJ-241947, is a novel peroxisome proliferator?activated receptor ? ligand [1]. The PPAR? receptors mainly express in adipose tissue, colon and macrophages involved in regulating fatty acid storage and glucose metabolism. |
 HIV-1 pol Integrase) Recombinant (E.Coli, His-tag) HIV-1 pol Integrase |
|
RP-555 |
Alpha Diagnostics |
10 ug |
EUR 164 |
 Alpha Fluor™ 488 amine |
|
1705 |
AAT Bioquest |
1 mg |
EUR 306 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 Alpha Fluor™ 488 Hydroxylamine |
|
1900 |
AAT Bioquest |
1 mg |
EUR 306 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 Tide Fluor 2-LL-37 |
|
H-8286.0100 |
Bachem |
0.1mg |
EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
Tide Fluor 2-LL-37 |
|
H-8286.0500 |
Bachem |
0.5mg |
EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
 Anti-Cytokeratins Alexa Fluor488 |
|
A4-108-C025 |
ExBio |
0.025 mg |
EUR 175 |
Anti-Cytokeratins Alexa Fluor488 |
|
A4-108-C100 |
ExBio |
0.1 mg |
EUR 310 |
 Anti-PSMA Alexa Fluor488 |
|
A4-539-C025 |
ExBio |
0.025 mg |
EUR 227 |
Anti-PSMA Alexa Fluor488 |
|
A4-539-C100 |
ExBio |
0.1 mg |
EUR 414 |
 Anti-FoxP3 Alexa Fluor488 |
|
A4-601-C025 |
ExBio |
0.025 mg |
EUR 201 |
Anti-FoxP3 Alexa Fluor488 |
|
A4-601-C100 |
ExBio |
0.1 mg |
EUR 362 |
 Anti-Phosphotyrosine Alexa Fluor647 |
|
A6-263-C025 |
ExBio |
0.025 mg |
EUR 154 |
Anti-Phosphotyrosine Alexa Fluor647 |
|
A6-263-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Anti-LCK Alexa Fluor647 |
|
A6-269-C025 |
ExBio |
0.025 mg |
EUR 206 |
Anti-LCK Alexa Fluor647 |
|
A6-269-C100 |
ExBio |
0.1 mg |
EUR 373 |
 Anti-FoxP3 Alexa Fluor647 |
|
A6-601-C025 |
ExBio |
0.025 mg |
EUR 201 |
Anti-FoxP3 Alexa Fluor647 |
|
A6-601-C100 |
ExBio |
0.1 mg |
EUR 362 |
 CellBrite Fix 555 |
|
30088 |
Biotium |
1KIT |
EUR 563 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 Cyanine 555 aminooxy |
|
96009 |
Biotium |
1MG |
EUR 342 |
|
Description: Minimum order quantity: 1 unit of 1MG |
 Cyanine 555 tyramide |
|
96020 |
Biotium |
0.5mg |
EUR 353 |
|
Description: Minimum order quantity: 1 unit of 0.5mg |
 Cyanine 555 alkyne |
|
92100 |
Biotium |
1MG |
EUR 226 |
|
Description: Minimum order quantity: 1 unit of 1MG |
) Monoclonal Anti-Monkey IgG-Alexa 555 Conj. (specific for monkey; no reactivity with human or animals IgG) |
|
70030-AF555 |
Alpha Diagnostics |
50 tests |
EUR 347 |
 Metal Fluor™ Zn-520, AM |
|
21263 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 Alpha Fluor™ 488 NHS Ester |
|
1812 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 Alpha Fluor™ 532 NHS Ester |
|
1819 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 Alpha Fluor™ 594 C5 Maleimide |
|
1891 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 Helix Fluor™ 6-JOE Phosphoramidite |
|
6046 |
AAT Bioquest |
100 umoles |
EUR 50 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
) Tide Fluor 2-LL-37 (scrambled) |
|
H-8288.0100 |
Bachem |
0.1mg |
EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
Tide Fluor 2-LL-37 (scrambled) |
|
H-8288.0500 |
Bachem |
0.5mg |
EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
 Tide Fluor 5WS-o-Conotoxin GVIA |
|
H-8356.0100 |
Bachem |
0.1mg |
EUR 1146 |
|
Description: Sum Formula: C120H182N38O43S6+dye |
 conjugate) Streptavidin-Alexa488 (Alexas fluor 488) conjugate |
|
SV-A488-100 |
Alpha Diagnostics |
100 tests |
EUR 225 |
 conjugate) Streptavidin-Alexa594 (Alexas fluor 594) conjugate |
|
SV-A594-100 |
Alpha Diagnostics |
100 tests |
EUR 225 |
 Anti-Cytokeratin 18 Alexa Fluor488 |
|
A4-106-C025 |
ExBio |
0.025 mg |
EUR 186 |
Anti-Cytokeratin 18 Alexa Fluor488 |
|
A4-106-C100 |
ExBio |
0.1 mg |
EUR 331 |
 Anti-Cytokeratin 19 Alexa Fluor488 |
|
A4-120-C025 |
ExBio |
0.025 mg |
EUR 186 |
Anti-Cytokeratin 19 Alexa Fluor488 |
|
A4-120-C100 |
ExBio |
0.1 mg |
EUR 331 |
 Anti-Ki-67 Alexa Fluor488 |
|
A4-155-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Ki-67 Alexa Fluor488 |
|
A4-155-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD45 Alexa Fluor488 |
|
A4-160-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD193 Alexa Fluor488 |
|
A4-161-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD279 Alexa Fluor488 |
|
A4-176-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD43 Alexa Fluor488 |
|
A4-220-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD43 Alexa Fluor488 |
|
A4-220-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD44 Alexa Fluor488 |
|
A4-221-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD44 Alexa Fluor488 |
|
A4-221-T100 |
ExBio |
100 tests |
EUR 269 |
Anti-Hu CD45 Alexa Fluor488 |
|
A4-222-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD45 Alexa Fluor488 |
|
A4-222-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD55 Alexa Fluor488 |
|
A4-230-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD55 Alexa Fluor488 |
|
A4-230-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD50 Alexa Fluor488 |
|
A4-266-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD50 Alexa Fluor488 |
|
A4-266-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD31 Alexa Fluor488 |
|
A4-273-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD31 Alexa Fluor488 |
|
A4-273-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD147 Alexa Fluor488 |
|
A4-274-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD147 Alexa Fluor488 |
|
A4-274-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD34 Alexa Fluor488 |
|
A4-297-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD34 Alexa Fluor488 |
|
A4-297-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD105 Alexa Fluor488 |
|
A4-298-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD105 Alexa Fluor488 |
|
A4-298-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD41 Alexa Fluor488 |
|
A4-309-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD41 Alexa Fluor488 |
|
A4-309-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD72 Alexa Fluor488 |
|
A4-310-T025 |
ExBio |
25 tests |
EUR 154 |
 Anti-Hu CD63 Alexa Fluor488 |
|
A4-343-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD63 Alexa Fluor488 |
|
A4-343-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD13 Alexa Fluor488 |
|
A4-396-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD13 Alexa Fluor488 |
|
A4-396-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-HLA-G Alexa Fluor488 |
|
A4-431-C025 |
ExBio |
0.025 mg |
EUR 217 |
Anti-HLA-G Alexa Fluor488 |
|
A4-431-C100 |
ExBio |
0.1 mg |
EUR 394 |
Anti-HLA-G Alexa Fluor488 |
|
A4-437-C025 |
ExBio |
0.025 mg |
EUR 217 |
Anti-HLA-G Alexa Fluor488 |
|
A4-437-C100 |
ExBio |
0.1 mg |
EUR 394 |
 Anti-Hu CD300a Alexa Fluor488 |
|
A4-501-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-HLA-A2 Alexa Fluor488 |
|
A4-556-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-HLA-A2 Alexa Fluor488 |
|
A4-556-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-CD3 zeta Alexa Fluor488 |
|
A4-568-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Anti-Ms CD8a Alexa Fluor488 |
|
A4-579-C025 |
ExBio |
0.025 mg |
EUR 139 |
Anti-Ms CD8a Alexa Fluor488 |
|
A4-579-C100 |
ExBio |
0.1 mg |
EUR 238 |
 Anti-Hu CD326 Alexa Fluor488 |
|
A4-582-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD3 Alexa Fluor488 |
|
A4-631-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD16 Alexa Fluor488 |
|
A4-646-T025 |
ExBio |
25 tests |
EUR 154 |
 Anti-Hu CD150 Alexa Fluor488 |
|
A4-660-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD107a Alexa Fluor488 |
|
A4-671-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD107a Alexa Fluor488 |
|
A4-671-T100 |
ExBio |
100 tests |
EUR 269 |
The survey revealed that 95% ofveterinarians scored mouse welfare as acceptable to wonderful, though areas for enchancment stay. These areas embody:1) coaching of researchers performing experimental procedures; 2) the frequency of monitoring mice more likely to experiencepain and misery because of experimental manipulation; 3) inclusion of the institutional veterinary workers within the monitoring ofmice more likely to expertise ache and misery; 4) continued enchancment within the environmental enrichment offered to mice;5) the power of the IACUC to make sure that cases of noncompliance are absolutely addressed with the intention to stop reoccurrence each inside laboratories and amongst different analysis teams on the establishment; and 6) reliance on non-veterinarians to carry out examinations, diagnose illness, and prescribe the remedy of sick or injured mice.